In this revised and expanded second edition, Electron Microscopy: Methods and Protocols presents the newest technology in electron microscopy, while maintaining the practicality and accessibility of the acclaimed first edition. Like the first edition, this volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. Each protocol is presented by experts with years of first-hand experience and in-depth knowledge of the techniques they describe. The Notes section accompanying each protocol will prove ibnvaluable for adapting and troubleshooting protocols.

Electron Microscopy: Methods and Protocols, Second Edition, comprises two major areas of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The TEM area covers several key techniques, including: conventional specimen preparation methods for cultured cells and biomedical and plant tissues; cryospecimen preparation by high-pressure freezing and cryoultramicrotomy negative staining and immunogold labeling techniques; and TEM crystallography and cryo-TEM tomography. The SEM area similarly attends to conventional- variable pressure-, environmental-, and cryo-scanning microscopy techniques, as well as the application of X-ray microanalysis. Protocols for the application of X-ray microanalysis to SEM and mass spectrometry conclude the volume.

Features─
1. Essential electron microscopy techniques for TEM and SEM.
2. Quantitative aspects of immunogold labeling in resin embedded
samples.
3. Examines the application of electron energy-loss spectroscopy
(EELS) to biomedical tissue.
4. Notes section enables efficient adaptation and troublehooting of
protocols.